2,4,5-T · Experiments

Pipeline experiments

Planned and available for reproduction, protocols and order files are included.

TR-FRETAvailableGenerator filter: passed

IVTT TR-FRET screen — 2,4,5-T gBlock constructs

Campaign: 2-4-5-t--bg-sm-denovo-f52-e2e · Target slug: 2,4,5-T

96-well IDT gBlocks plate. Construct: T7 promoter + GG + SD + Kozak + ATG + FLAG (DYKDDDDK) + E. coli MFC CDS + TAATAG (2x stop) + GTCGAC + T7 terminator (SOP 03). QC: 20/20 valid per gblock_qc.tsv.

Binders measured

Top WeaveRank

1.52

Generator filter

20/20 passed

1BG_SM_DENOVO_F52_E2E_boltzgen_binder_019boltzgen1.52
2BG_SM_DENOVO_F52_E2E_boltzgen_binder_008boltzgen0.95
3BG_SM_DENOVO_F52_E2E_boltzgen_binder_005boltzgen0.78
4BG_SM_DENOVO_F52_E2E_boltzgen_binder_006boltzgen0.55
5BG_SM_DENOVO_F52_E2E_boltzgen_binder_012boltzgen0.05

Procedure & files

Vector

linear gBlock template (T7-SD-Kozak-FLAG construct)

Protocols

Materials

IDT gBlocks (96-well plate)
TnT T7 Quick IVTT kit
HTRF anti-FLAG reagents

Conditions

codon_table: E. coli MFC
construct_context: T7+GG+SD+Kozak+ATG+FLAG+CDS+TAATAG+GTCGAC+T7-terminator

Order files

2-4-5-t--bg-sm-denovo-f52-e2e_gblocks_plate.xlsx

Position	Candidate	Sequence	Tool	WeaveRank
A1	BG_SM_DENOVO_F52_E2E_boltzgen_binder_019	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCCGGATACCGTGGTGAAAGTGATTCTGACCGATGAAACCGGCATTCTGCCGAACAAAGAAGTGGAAATGACCGTGGCGGAAGCGGTGGAAAAAATTAAAGAAGCGCGTGCGAAAGTGCCGGAAGATGAAGAAATTCTGCTGAACGGCGTGCTGATTAGCAAAGATGCGAGCGATGAAGAACTGATGGCGCTGTTTAACGCGGCGGGCGTGGTGGATGTGGTGGTGAAAAAAAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	1.52
A2	BG_SM_DENOVO_F52_E2E_boltzgen_binder_008	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGCCGGTGGTGAAAACCATTCTGGTGCGTGGCCTGACCGCGGCGGAACTGGAACGTATTCGTCGTGCGCTGCAGGCGAACACCCCGTTTTTTAGCGAAAAACTGCAGCGTGCGGGCGCGGGCAAACTGGTGCGTATTCTGGGCGTGCGTCATACCGAAACCGAAGCGGAACTGGTGGTGGAAGATCTGGAACAGACCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.95
A3	BG_SM_DENOVO_F52_E2E_boltzgen_binder_005	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCCGCCGCCGCGTGTGGTGACCACCTTTCTGAAAAAAAGCCATAAAGAAGCGGTGGAAAAAGTGGAAAAACTGGAACCGGGCGGCGAAGCGGTGATTGATGGCAGCGATGTGACCAACCTGGAAGGCACCGCGCGTGTGCGTCTGCTGGATTGGCGTGAAGTGCCGGATGGCATTGAACTGCGTGTGGAAGTGCTGGAAGTGCGTTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.78
A4	BG_SM_DENOVO_F52_E2E_boltzgen_binder_006	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCCGAAAGTGATTGAAGTGACCGTGACCGAAGAACGTATTAAAATTCTGCGTGAAGCGCTGAAAATGCTGGAACAGGGCGCGAGCAACGAAGAAATTAAAAAATATCTGGCGGAAAACAGCCCGCAGGGCGCGAAAGTGCTGAGCGAAGCGGAATTTACCAAAGAACAGGCGAAACCGCTGCTGAAAAAAGCGACCAGCCTGCCGCCGGGCAGCACCTTTACCCTGGAATTTAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.55
A5	BG_SM_DENOVO_F52_E2E_boltzgen_binder_012	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGTGACCGAAGAAATGCTGGAAAAAGCGCGTCGTCTGAAAGAACATATTGAACGTGCGCTGGCGAAAGGCGAAACCGAAGAAGTGCGTGCGGAACTGCTGGCGTATCTGATTAGCGAAGGCTTTACCCGTGAACAGGCGGAAAAAGTGATTGAACTGGTGAAAGCGGGCGCGAGCGTGGAAGAAATTGCGGAAGCGCTGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.05
A6	BG_SM_DENOVO_F52_E2E_boltzgen_binder_003	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCTGAGCAGCCTGACCCCGGAACAGAAAGAAAAACTGGTGAACGGCCTGAAAAGCAAAAGCAACGAAGAACTGCAGAGCATGAGCCGTGAAGAAATTCTGCGTATGCTGCGTGAACAGGGCCTGAGCGAAGAAGTGGCGGAAGAACTGTATGATATTTTTGAAAAACATGGCACCAGCGAAGCGGTGATTGAACTGCTGAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.01
A7	BG_SM_DENOVO_F52_E2E_boltzgen_binder_004	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCAAAGTGGTGGTGACCGCGAAAGTGGCGCCGGAAGATAACCCGAACAAATGGTATGAACTGAAAGGCGAAATTGTGAGCGAAGATGAAGATGGCAAATATGTGACCATTTATTTTCTGGAATTTAAAGTGACCAAAACCATTCCGGTGAGCCGTATTCTGAAAGTGGAAATTGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.01
A8	BG_SM_DENOVO_F52_E2E_boltzgen_binder_017	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCGAAGAAGAACTGAAACAGCTGCTGCTGAAAAACGGCCAGGCGATTCTGGAACTGCTGGAAGCGAACACCGATCTGAGCGATGAAGAACAGAAAGAACTGGCGAAAAAATATATTGAAAAAGCGTTTAAAGATTATCCGGAAACCCGTGAAAAACTGCTGAAAGAACTGAACGAATGCAAAAGCCGTGAAGAACTGATTGAACGTATTCGTGAAGCGATTAAAGAATATAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.04
A9	BG_SM_DENOVO_F52_E2E_boltzgen_binder_002	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAAAGCGCCGGATGCGGTGCTGAGCCGTGAAGAAGCGGAAGAACTGATTCGTCATACCGCGGAACGTCTGCTGGCGGAAGGCGTGCCGCTGGAAAAAGTGCTGGCGGCGGCGCGTGCGATTCGTGAACTGGCGAAAGATGGCACCGTGACCGTGCAGCAGATTATTGATGCGATTAGCAAAGAAACCGGCCTGAGCCGTGCGCGTATTGAAGCGATTCTGACCGGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.11
A10	BG_SM_DENOVO_F52_E2E_boltzgen_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGAAAAACGTGTGGTGTATGCGAAAGGCACCAACGCGGGCAAATATACCGGCAAATGGTTTAAAGCGCTGGTGCTGAAAGAAACCGAAGAAACCGCGGAAGTGGAAGTGCTGGAAGGCCCGGCGAAAGGCGTGGTGGGCGAAGTGCCGAACAAAAACATTTTTGATGAAAAACCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.14
A11	BG_SM_DENOVO_F52_E2E_boltzgen_binder_020	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGAAATTTACCCCGCAGCAGAAAGCGCTGCTGGCGGTGCTGAACGAAGTGAAAGCGAAAGTGCCGGAAGATGATAAAGAAGCGCTGGCGCTGCTGGAAAAAGCGGGCAAACTGCTGGCGGAAGGCAAATATGAAGAAGCGGAAAAACTGCTGCTGGAAGCGGCGAAACTGGTGGGCGTGGAAGATGAAGCGGAAGCGCGTATTGCGGCGATTCGTTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.23
A12	BG_SM_DENOVO_F52_E2E_boltzgen_binder_007	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCCGACCGAAGTGCCGAAAACCGAAGAAGAACGTCGTGCGGCGGTGCGTGAACGTGTGCGTAAAATGCTGGCGGAAGGCAAAAGCGAAGAAGAAATTATTGAAGAACTGAGCAAATATGGCTTTGGCGAAGAAGCGCGTGAAGTGATTAAAGAACTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.24
B1	BG_SM_DENOVO_F52_E2E_boltzgen_binder_015	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCCGGAAGTGGGCCAGGTGGTGGCGGTGCGTCCGCTGCGTGATCCGAGCAAAGTGTATCGTGCGAAAGTGCTGAGCAAAGATCCGAACGATCCGAGCATTTATAAAGTGGAATTTCTGGAAAACGGCGAAGTGGGCGATGTGCCGGTGAAAAACATTATTGGCCCGGCGACCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.36
B2	BG_SM_DENOVO_F52_E2E_boltzgen_binder_016	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGTGCCCGCCGGGCAAACTGCCGATTGTGAAAGCGCTGAAAGATTATGAAGCGAAAACCCCGGATGAACTGAGCTTTAAAAAAGGCGAACTGCTGCTGGTGCTGGCGTTTAGCGAAGATGGCAAATGGGCGCTGGTGAAAAGCCTGGAAACCGGCAAAGTGGGCAAAGTGCCGACCAGCTATGTGGAAGTGGTGGGCTGCGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.42
B3	BG_SM_DENOVO_F52_E2E_boltzgen_binder_014	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCCGAAAGTGCTGAAAGTGAAACTGACCGCGGAAGATGGCAAAGTGCTGACCTGCACCGAAAAAGTGCTGGAAGAAACCGAAACCAAACGTGTGGCGGAAGTGACCGATGATGAAGGCCGTAAAGATCGTGTGACCCTGGAAATTCTGGAAAAAGATGGCGATGTGTGGAAAGCGAAACTGACCGCGGAAAGCGGCAAAGTGGTGACCGTGACCATTGAAGTGCTGGAAGTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.48
B4	BG_SM_DENOVO_F52_E2E_boltzgen_binder_009	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGAGCGAACTGGTGGAAGTGGTGCTGAAACTGAGCGAAGAAGAACGTAAAAAAATTCTGAACAAAGTGAAAAGCCTGACCGAAGAACAGAAAAAAGAACTGCTGGAAATTGCGGAACAGCTGAAAAGCGGCAAAATTAGCGCGGAAGAAGCGGTGGAACGTGTGAAAGAAAAATTTAGCGAAGAAGTGATTAAAGAAGTGGGCGAACTGGTGCTGAAAAGCAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.56
B5	BG_SM_DENOVO_F52_E2E_boltzgen_binder_010	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCAAAAAAATTGTGAAAGCGAAAAAAGATTATGAAGGCCCGGCGAGCCTGAAAAAAGGCGATCTGCTGGAAGTGCTGGAAGAAACCGAAGAACCGGGCTGGTGGCTGGTGAAAAACCTGGAAACCGGCGAAGTGGGCAAAGTGCCGAAAAAATATGTGGAACTGGTGGGCGAAAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.62
B6	BG_SM_DENOVO_F52_E2E_boltzgen_binder_013	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCCGCGTCTGGTGGTGGTGGCGCTGAAAGATTATGTGGGCGGCGTGACCTTTAAAAAAGGCGATAAACTGCTGGTGCTGGGCGAAAGCGAAGTGCCGGGCCTGTGGCGTTGCCAGCTGCTGGAAACCGGCGAAGTGGGCCTGATTCCGAAAAAAGATGTGAAAGTGGTGGGCGAAGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.64
B7	BG_SM_DENOVO_F52_E2E_boltzgen_binder_018	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGCGCCGCGTCCGGTGGTGCGTGCGCTGCGTGATTATACCGGCACCAACAGCGATGGCCAGACCCTGACCGCGAAAGTGGGCGATAAACTGCTGGTGCTGGATGATAGCAACCCGGATTGGTGGCTGTGCCAGAGCCTGGAAACCGGCCAGGTGGGCTGGGTGCCGAAAAAAGATGTGGAAGTGGTGGGCACCGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.67
B8	BG_SM_DENOVO_F52_E2E_boltzgen_binder_011	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGCCGGCGGAAGTGGGCCAGCGTGTGAAAGTGAAAAGCAAAGATGGCAAAGAATATCTGGCGGAAGTGGAAGAAGTGGATGGCGATATGGCGCTGGTGAAATTTCTGGAAACCGGCAAACGTGAATGGGTGAAAATTACCAAAGAAGATCTGGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.79

Yeast Surface DisplayAvailableGenerator filter: passed

Yeast surface display screen — 2,4,5-T binder oligo pools

Campaign: 2-4-5-t--bg-sm-denovo-f52-e2e · Target slug: 2,4,5-T

IDT oPools order, tiered by confidence: tier1_50pmol (20 oligos). Gap-repair cloning into pCTCON2.

Binders measured

114

Top Pool score

0.82