2,4-D · Experiments

Pipeline experiments

Planned and available for reproduction, protocols and order files are included.

TR-FRETAvailableGenerator filter: passed

IVTT TR-FRET screen — 2,4-D gBlock constructs

Campaign: 2-4-d--bg-sm-denovo-cfa-e2e · Target slug: 2,4-D

96-well IDT gBlocks plate. Construct: T7 promoter + GG + SD + Kozak + ATG + FLAG (DYKDDDDK) + E. coli MFC CDS + TAATAG (2x stop) + GTCGAC + T7 terminator (SOP 03). QC: 20/20 valid per gblock_qc.tsv.

Binders measured

Top WeaveRank

1.24

Generator filter

20/20 passed

1BG_SM_DENOVO_CFA_E2E_boltzgen_binder_006boltzgen1.24
2BG_SM_DENOVO_CFA_E2E_boltzgen_binder_002boltzgen0.43
3BG_SM_DENOVO_CFA_E2E_boltzgen_binder_016boltzgen0.37
4BG_SM_DENOVO_CFA_E2E_boltzgen_binder_019boltzgen0.31
5BG_SM_DENOVO_CFA_E2E_boltzgen_binder_014boltzgen0.26

Procedure & files

Vector

linear gBlock template (T7-SD-Kozak-FLAG construct)

Protocols

Materials

IDT gBlocks (96-well plate)
TnT T7 Quick IVTT kit
HTRF anti-FLAG reagents

Conditions

codon_table: E. coli MFC
construct_context: T7+GG+SD+Kozak+ATG+FLAG+CDS+TAATAG+GTCGAC+T7-terminator

Order files

2-4-d--bg-sm-denovo-cfa-e2e_gblocks_plate.xlsx

Position	Candidate	Sequence	Tool	WeaveRank
A1	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_006	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGCGGCGGCGGAACGTGCGGCGCTGGTGGAACGTCAGCGTGCGCTGATTGAAGAAGTGAAAAAAGAACTGGATCCGAAAGATGAACTGGCGCGTCGTGCGGTGCGTGTGATTCGTAAAAAAGCGGAAGCGGGCGAAATTAGCCTGGAAGAATGCGAAGAAATTTGCAAACTGCTGAAAGCGGGCAAATATGAAGAAGCGCTGAAAAAACTGGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	1.24
A2	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_002	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGGTGGGCGATATTGTGGTGGCGCTGAAAGATTATACCGCGAAACGTCCGGATGAACTGAGCTTTAAAAAAGGCGAACGTCTGCTGGTGCTGGCGGAAAGCCCGGATGGCAGCCGTTGGCTGGTGCAGAGCCTGGAAACCGGCGAACGTGGCCTGGTGCCGACCAGCTATGTGAAAGTGGTGGGCCGTGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.43
A3	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_016	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGCTGGTGAAAGCGCTGAAAGATTATACCGCGACCAAACCGTATGAACTGAGCTTTAAAAAAGGCGATATTCTGGAAGTGCTGGAAATTCTGAACGATGAATGGGTGCTGGCGAAAAGCCTGAACACCGGCGAAGTGGGCGTGGTGCCGGCGAAAAACGTGGAAGAAATTGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.37
A4	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_019	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCAGCAGCGATACCACCGTGTATGTGGCGCTGAAAGATTATACCGCGACCAAACCGAACGAACTGAGCTTTAAAAAAGGCGATATTCTGCAGGTGCTGGATGATAGCCATGGCGATTGGTGGCTGGCGCGTAGCCTGAACACCGGCAAAACCGGCTGGATTCCGAGCAAAAACGTGAAAGAAGTGCCGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.31
A5	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_014	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCAGCCTGAAAGAATATCTGCGTGAACTGGTGGAAGAAAAAGTGAGCGGCGAAACCCGTGAACGTATTCTGGCGGCGATTGAAGCGGATCGTCTGAGCCGTGAAGGCGCGCTGCTGGTGGCGGATCTGCTGGAAGAAGAAGGCCAGGAAGAACTGGCGCGTTGCCTGCGTGAAGCGGCGGAAAAATTTGTGGATGAAGATGGCTTTATTAGCCTGAGCGATATTGAAAAATATTGCAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.26
A6	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_013	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCGCGGCGGCGGCGGCGGCGGCGCGTCGTGAAGAACTGGTGGCGCGTCTGGTGGAAGAACTGAAAGATCCGGCGAAACGTAACGAAGAACATATTAAAGCGCTGGTGAAAGAAGCGAAAGCGGCGGGCCTGAGCTATGCGGAAATTGCGGAAGCGCTGACCCGTGTGGGCATTCCGCGTACCGTGGCGGAAGTGGAAAAAATGGCGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.24
A7	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_011	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGCGCTGGTGGCGAAAAAAGATTATATTGCGACCAGCCCGGATGAAATTAGCGTGAAACCGGGCGATAAAGTGCTGGAAGTGGTGGATGATGAATGGGTGGTGGTGCAGAACAGCGATGGCCGTACCGGCCTGGCGCCGCGTAAATATTGCGAAGAAAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.19
A8	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_003	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGAAATTTGTGGCGACCAAAGATTATACCGCGAAAACCGCGGATGAAGTGAGCGTGAAAAAAGGCACCGAAGTGCTGGATAAACCGAGCGAAGATTGGGTGGTGGCGAAAGGCCCGATTACCGGCAAAGAAGGCCTGATTCCGGCGAAATATGTGGAAGAAGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.16
A9	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGGAAGCGAAAGTGGGCGATGTGGTGCTGGTGAAAACCCTGGATGGCGAAGTGCGTCGTGCGCGTGTGGTGGAAATTCTGGATGAAGATACCGTGCTGGTGGAACTGCTGGATACCGGCCTGCGTGGCGTGCTGCCGAAAAGCCGTATTCTGGAAGTGCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.14
A10	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_010	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCGTCTGAAAAAATTTAAACTGCGTTATCGTGATCGTGATGGCGAATGGCGTGAAGTGGAAGTGGAAGCGACCAGCGAAGAAGAAGCGAAAGAAAAAGTGCATGAAGAATATGGCGTGCCGAAAGAAGATATTGAAGTGCTGAGCATTGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.10
A11	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_008	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGAAAATTATTGCGAAAGCGCTGAAAGATTATGAAGGCGAACATGCGATTAAAAAAGGCGATATTATTGAAATTCTGGAACAGGATGGCAACAAAGCGCTGGTGAAAAACCTGAACACCGGCAAAGAAGGCTGGATTGATCTGAGCAAAATTGAATTTGAAGTGCTGGCGGTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.09
A12	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_004	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGAAAGTGGGCGATGTGGTGCTGGCGCAGACCAAAGATGGCCGTCGTGGCGTGGCGATTGTGGCGAAAGTGCTGAGCGATGATGTGGCGGTGGTGGGCGAAGTGGTGGAAGGCGATCTGGAAGCGGGCGAACGTGTGCGTATTCTGGCGAAAGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.06
B1	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_020	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCCTGGAAGGCACCGGCGTGTATGTGTTTAAAACCAAAGATGGCAAAGTGTTTAAAGTGGAAGCGACCAACCTGACCGAAGAAGAAGCGAAAGAACTGGCGGGCGAAAAATATGGCATTCCGCCGGAAGATCTGGAACTGCTGGGCAAAGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.16
B2	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_012	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCAGCGTGGGCCTGGCGCGTGCGCTGGTGGATTATACCGCGAAAACCCCGGAAGAACTGAGCTTTAAAAAAGGCGAACTGCTGGAAGTGCTGGCGGTGAGCCCGGATGGCAAATGGCTGCTGGCGAAAAGCCTGGAAACCGGCGAAGTGGGCCGTGTGCCGGCGAGCCATGTGGAAATTCTGAGCCCGATTTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.29
B3	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_015	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGAAACCCTGATTACCCCGAGCCCGCTGCTGCTGAAACTGCTGAAAAGCGCGGGCGCGCAGAAAGATACCTATACCATGAAAGAAGTGCTGTTTTATCTGGGCCAGTATATTATGACCAAACGTCTGTATGATGAAAAACAGCAGCATATTGTGTATTGCAGCAACGATCTGCTGGGCGATGTGCTGGGCGTGCCGAGCTTTAGCACCAAAGAACATAGCAAAATTTATAGCATGCTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.32
B4	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_017	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGGTGGTGACCTTTACCACCAAAAGCGGCAAAACCGTGACCCTGGAAGTGCGTCCGAGCGATACCGTGCTGCGTATTGCGGGCGAAATTGCGCGTCAGCTGGGCATTCATCCGAGCGAAGAAGAACTGAAAGCGATTCTGGAAAGCCTGGGCATTGATCCGGATGCGGTGCCGCGTCTGAGCCTGGAAGAACTGGAAAAACTGGCGCAGAAAATTGCGGATCTGCTGGAAGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.32
B5	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_007	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCAAAGTGAAAGTGTATCTGGTGCTGGTGCGTGATAAAAACGGCAAAGTGCATGAAATTGAAGTGGAAGCGACCAGCGAAGAAGGCGCGAAAGAACGTGCGAGCGAACTGACCGGCGCGCCGAAAGAACGTATTCGTGTGCTGAGCGCGCGTTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.51
B6	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_005	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGCGGGCGAAGTGGTGACCGGCAAAATTGTGGCGAAAAGCCCGGATGGCAAAACCGCGCTGGTGCTGCTGGAAAACGGCGAACGTGCGCTGGTGAAAAACTATAAAGTGGGCGAACCGGCGGAAGCGGTGCTGGAAGATGGCCGTAAAGCGAAAGTGCTGAGCGCGACCGAAACCACCGTGACCGTGCAGATTCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.59
B7	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_009	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCTGCCGAAAATTGAACTGACCCCGGAACAGATTGAAGCGGCGCGTGCGGCGGTGGAACATATTCTGGCGAACCCGAGCCTGACCTTTGAAGAAATGATTGCGTATTTTGTGGCGCAGGGCCTGAGCAAAGAAACCGCGGAAATTCTGGCGAAAATGGTGATGGATGGCGCGAGCCTGGAAGAAATTCTGGCGAGCCTGGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.72
B8	BG_SM_DENOVO_CFA_E2E_boltzgen_binder_018	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGCGCCGGTGACCCTGACCGTGGAAGTGAAAGCGGCGGATGAAGAAACCCTGGATGTGACCGTGACCACCGAAGATGGCACCGTGCTGATTGAAGGCCGTTATAAAGTGGAAGAAGTGATTAAAAAACTGGTGGCGGCGGGCATTAGCGAAGAAGGCGCGCGTCGTCTGGTGGAAGAAGCGGTGAAAAACACCGGCAAACATACCTTTACCGTGGCGCGTGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.80

Yeast Surface DisplayAvailableGenerator filter: passed

Yeast surface display screen — 2,4-D binder oligo pools

Campaign: 2-4-d--bg-sm-denovo-cfa-e2e · Target slug: 2,4-D

IDT oPools order, tiered by confidence: tier1_50pmol (20 oligos). Gap-repair cloning into pCTCON2.

Binders measured

110

Top Pool score

0.79