DDE · Experiments

Pipeline experiments

Planned and available for reproduction, protocols and order files are included.

TR-FRETAvailableGenerator filter: passed

IVTT TR-FRET screen — DDE gBlock constructs

Campaign: dde--bg-sm-denovo-6ws-e2e · Target slug: DDE

96-well IDT gBlocks plate. Construct: T7 promoter + GG + SD + Kozak + ATG + FLAG (DYKDDDDK) + E. coli MFC CDS + TAATAG (2x stop) + GTCGAC + T7 terminator (SOP 03). QC: 20/20 valid per gblock_qc.tsv.

Binders measured

Top WeaveRank

1.32

Generator filter

20/20 passed

1BG_SM_DENOVO_6WS_E2E_boltzgen_binder_008boltzgen1.32
2BG_SM_DENOVO_6WS_E2E_boltzgen_binder_018boltzgen0.72
3BG_SM_DENOVO_6WS_E2E_boltzgen_binder_001boltzgen0.60
4BG_SM_DENOVO_6WS_E2E_boltzgen_binder_003boltzgen0.54
5BG_SM_DENOVO_6WS_E2E_boltzgen_binder_002boltzgen0.44

Procedure & files

Vector

linear gBlock template (T7-SD-Kozak-FLAG construct)

Protocols

Materials

IDT gBlocks (96-well plate)
TnT T7 Quick IVTT kit
HTRF anti-FLAG reagents

Conditions

codon_table: E. coli MFC
construct_context: T7+GG+SD+Kozak+ATG+FLAG+CDS+TAATAG+GTCGAC+T7-terminator

Order files

dde--bg-sm-denovo-6ws-e2e_gblocks_plate.xlsx

Position	Candidate	Sequence	Tool	WeaveRank
A1	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_008	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCATGCTGAGCGGCAAATGGCTGGCGACCGCGGATAGCGGCACCGTGGATGTGGTGACCCTGACCGTGCTGAGCGAAGATATTCTGGAAGCGACCTTTGAAGATGGCAAAAAAGTGCTGCTGAAAGTGAGCAACCTGCAGGTGGATGGCGATGTGGTGACCTGCGATGCGGAAATTCTGAGCGGCGAAAAACGTAGCGGCAAAGTGACCCTGACCCGTATTTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	1.32
A2	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_018	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCGAAGAACAGCTGAAAGAAGCGGAAGAACAGCTGCGTAAACTGGGCCGTGAATATATGGAAAAATATGGCGTGCCGGAAGAAACCCGTCGTAAACTGATTGAAGAAGCGATTAAACTGCTGCGTAAAAAAAAATTTGAAGAACTGGAAGAACGTATTCGTGAAGAAGTGAGCGATCCGGAAATTCTGGAAGAAGCGCTGCAGCTGGTGAAAAAACTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.72
A3	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCGAAGCGGTGCTGAAAAAAGCGGAAGAAACCCTGAAAGAAATGCAGACCCTGGGCAACGATGTGGCGCGTATTCGTGCGCTGCTGAGCACCCTGAGCGATGAAGAACGTGAAGCGCTGATTGAACTGGCGAAAAAAGAAAGCAAAGAAATTGCGGAAAAACTGAAAAAACTGTATGAAGAACTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.60
A4	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_003	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCAGCATTGATGGCGAATGGAAAGTGATTGAAAGCACCCATGTGCCGGTGGGCACCATTCTGAAAATTACCCAGGTGGCGCCGGGCGTGTATGAACTGACCAGCCTGGAAACCGGCGAAACCGCGACCGGCACCAACATTAAAGTGGATGGCGATATTCTGACCCTGACCGTGACCAAAGGCAGCAAAACCGCGACCGTGAAACTGGAAAAAATTTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.54
A5	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_002	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGGTGGAAACCTTTGAAGCGAACGTGACCAGCGTGGAAACCGGCGAAAACGCGGGCCTGGTGAAAGTGACCCTGGATGATGAAAAAAACACCTTTACCGTGGAAAACGAAGAAGTGAGCGCGACCGTGGGCGATATTCAGCGTGATGGCGATGTGCTGACCGGCACCGTGACCGAAGGCCCGGCGGTGGGCAAACGTATTAAAATTACCAAAGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.44
A6	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_010	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCCGCTGGAAGAAGCGATTCGTCGTGCGGTGGAACTGCTGAAAAAAGGCAAATATGAAGAAGCGGTGGCGGTGATTAAAGAAGTGGGCCCGACCGAAGAAGTGCTGGAAGCGCTGCGTGAAAACGCGCCGGAAGAAGCGTTTGAAAAAGTGGAAGAAGCGCTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.36
A7	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_004	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCAAATATAGCGGCGAATGGCTGGTGACCACCAGCAGCGGCCTGGTGGCGAAAGCGACCGTGACCGTGCTGGAAGAAACCGAAGATAAAATTATTCTGGAACTGAAACTGGAAGATGGCACCGTGGTGAAAGCGGAAGTGCTGAAACAGGATGGCGATGTGCTGGATGTGCGTTATACCGCGAGCAGCGGCGAAACCGGCGAAGATACCGTGACCCTGACCAAACTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.20
A8	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_007	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCCGAAAGTGATTACCGCGAAAATTAAAGTGGAAAAAATTGATAAAGAAAAACGTACCGCGGAAGCGACCGTGACCTTTGAAGATGGCAAAGAAAGCAAAGCGACCATTACCGTGGATCCGGAAGATCCGAGCCTGTATACCCTGACCCTGGATGAAACCGGCGAAAAATATAGCTTTAAAAAAGTGGAACTGAAACTGGGCGATGAAGCGACCGTGACCCTGACCCAGCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.04
A9	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_005	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGTGGATTTTAGCGGCAAATGGAAAGTGACCGCGAGCAGCGGCATTGTGGGCACCTGGGAAATTACCAAAATTAGCGAAAACACCCTGAAAGTGAAAACCGTGGATGGCAGCGTGCCGGAACGTACCGCGAAAATTCTGAGCCTGGAAGTGGATGGCGATGTGGTGACCCTGACCGTGGAACTGGATGATGGCCGTGTGTTTACCTTTAAACTGACCAAAATTTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.03
A10	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_009	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGCCGCCGGAAGTGAAAGTGGGCCAGGAAGTGGAAGCGAAAGCGCGTGATGGCCGTTGGTATAAAGCGAAAGTGGTGGAAGTGGATAAAGAAAAAGAAACCGCGCTGCTGAAATTTCTGGAAACCGGCGAAGAAGCGTGGGTGCCGCTGAGCCGTCTGCGTCCGCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.08
A11	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_020	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCTTTAGCCCGGAACTGAAAGAAAAAATTCGTAAAGCGGTGGAATATCTGGTGAAAGAAAAAGGCCTGGGCCGTGATGGCCGTGTGAGCAAAGCGGAATTTGCGGCGGTGCTGGAAGCGCTGGGCGTGCCGGCGGAAGTGGTGGATGCGTTTGATAAAGATGGCGATGGCACCGTGAGCGTGGGCGATGCGTATAACGCGGTGAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.21
A12	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_015	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGGAAGGCGTGGTGAAACTGCGTGGCAAAGTGCTGGAACAGGAACTGAAAGAAGATAGCGCGGAAGCGACCGTGGAATTTGAAGATGGCCGTGTGAGCAAAGTGAAAATTACCAAACTGCCGGATGGCAGCCTGCTGGCGACCCTGCTGGAAACCGGCGAAAAATATCGTGTGCTGAAACTGAAACAGCTGAGCGAAAAAGAACTGGAACTGGAAGTGAAACGTGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.23
B1	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_019	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCAGCCTGACCCCGGAACAGAAAGAAGAACTGCGTACCTTTGCGCGTAAAATGAAAGCGGCGGGCCTGAGCGATGAAGAAATTATTAAACGTCTGAAAGAATATTGCAAAAAAAAAGGCATTAACGCGACCGATGCGGAAATTCGTGAAGTGCTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.23
B2	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_017	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCATTGCGGATCTGACCCCGGAACAGAAAGAACGTATTCTGAACGGCCTGCGTCGTTATCTGGAAGCGCGTGGCCGTGATAGCGTGACCCCGGAAGAACTGAAACGTATTATGCTGGCGGCGGGCGCGACCGAAGAAACCGCGGATGAAGTGATTAGCCTGTTTCCGGGCGAAGTGACCGTGGAAGAACTGAAAGAAGCGCTGAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.24
B3	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_013	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGAAAAAATGCAAACTGATTCTGAAAGATAGCAAAGTGGTGAAAGATGGCGAAACCGCGGAAGTGGAAGTGATTGCGGAAGATGGCAGCACCGGCACCGGCAAACTGACCGTGGAAGGCGATACCTTTACCAACCCGGAACTGGGCGCGACCGCGGAAGGCGTGACCGAACTGCGTGAAGGCGATGTGCTGGAAGTGACCCTGGCGGATGGCAGCACCTATAAAGCGGAAGTGGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.32
B4	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_006	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCAGCCTGCCGCTGATTCCGGGCAGCCAGATTAGCGATGTGAAAAAAGAAGGCGATAAAATTCTGTTTAAAGGCACCTATAAAGGCAAAACCCATACCTTTGAAGTGGTGGAACTGCGTAAAGGCGATTATGTGGTGGTGAAAGCGCTGGAAGATGGCAGCCAGGCGGCGGTGAGCGTGAAAGATCTGATTGCGGCGCTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.34
B5	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_012	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGGTGGGCGTGTATAAATGCAAAATTAAAACCCCGAGCGGCGAATGGAAAGAACTGATTGCGGAAATTCTGGAATATAACGAAGAAAAAGGCAAAGCGAAAATTAAAAACCTGGAAACCGGCGAAGAAGCGGTGGTGCCGCTGAAAGATGTGAAAATTATTAAAAAAGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.55
B6	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_011	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGAGCGAAGATCTGCTGGGCACCTGGAACGTGACCTTTAGCAGCGGCAAAACCTATACCTTTACCGTGACCAGCGTGGATGGCAACACCATTACCGGCACCTTTACCGATGAAAGCGGCGAAACCTATACCGTGGTGATTGAAAACTTTGATGTGGCGACCGGCAAAGGCACCGCGAAAGTGTATAAAGGCGATGAACTGGTGGCGGAAGGCGAAGTGACCCTGACCCCGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.72
B7	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_016	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCGTCCGAAACTGATTGAAGCGACCATTAACGATCCGACCGGCGTGGTGAAAAACGGCCGTCATCTGCTGGAAGTGCTGGCGGAAGATGAAGATCGTGTGCTGGTGAAAAACCTGGAAACCGGCGAAGTGAAATGGATTCCGAAAAGCGCGCTGGAAGATGTGAAAGAAGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.76
B8	BG_SM_DENOVO_6WS_E2E_boltzgen_binder_014	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCATTCTGGGCACCTATAAATGCCTGGTGACCGCGGAAGATGGCAGCCAGACCGAAGTGCTGATGAAAGTGCTGGAAGTGGATGGCAACGAAATTGTGTTTCAGCTGGGCGATAAAACCTTTAAAGCGAAACTGCTGAAAGGCGATGAATTTAAAGTGGGCGCGACCGTGGAACTGGAAGTGGAAGATGAAAACGGCAAAAAAGTGACCCTGGAAGTGACCGTGCTGGAAAAACTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.89

Yeast Surface DisplayAvailableGenerator filter: passed

Yeast surface display screen — DDE binder oligo pools

Campaign: dde--bg-sm-denovo-6ws-e2e · Target slug: DDE

IDT oPools order, tiered by confidence: tier1_50pmol (20 oligos). Gap-repair cloning into pCTCON2.

Binders measured

120

Top Pool score

0.77