Saxitoxin · Experiments

Pipeline experiments

Planned and available for reproduction, protocols and order files are included.

TR-FRETAvailableGenerator filter: passed

IVTT TR-FRET screen — Saxitoxin gBlock constructs

Campaign: saxitoxin--bg-sm-denovo-stx-e2e · Target slug: Saxitoxin

96-well IDT gBlocks plate. Construct: T7 promoter + GG + SD + Kozak + ATG + FLAG (DYKDDDDK) + E. coli MFC CDS + TAATAG (2x stop) + GTCGAC + T7 terminator (SOP 03). QC: 20/20 valid per gblock_qc.tsv.

Binders measured

Top WeaveRank

0.73

Generator filter

20/20 passed

1BG_SM_DENOVO_STX_E2E_boltzgen_binder_016boltzgen0.73
2BG_SM_DENOVO_STX_E2E_boltzgen_binder_002boltzgen0.42
3BG_SM_DENOVO_STX_E2E_boltzgen_binder_020boltzgen0.35
4BG_SM_DENOVO_STX_E2E_boltzgen_binder_011boltzgen0.33
5BG_SM_DENOVO_STX_E2E_boltzgen_binder_001boltzgen0.30

Procedure & files

Vector

linear gBlock template (T7-SD-Kozak-FLAG construct)

Protocols

Materials

IDT gBlocks (96-well plate)
TnT T7 Quick IVTT kit
HTRF anti-FLAG reagents

Conditions

codon_table: E. coli MFC
construct_context: T7+GG+SD+Kozak+ATG+FLAG+CDS+TAATAG+GTCGAC+T7-terminator

Order files

saxitoxin--bg-sm-denovo-stx-e2e_gblocks_plate.xlsx

Position	Candidate	Sequence	Tool	WeaveRank
A1	BG_SM_DENOVO_STX_E2E_boltzgen_binder_016	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCCGTTTCGTGTGGGCGATGTGGTGCTGGCGAAAGGCAGCGATGGCAAATGGTATAAAGCGGAAGTGCTGGAAGTGCTGGATGAAGAACATGTGCTGGTGAAATTTCTGGAAACCGGCGAAGAACAGGTGGTGCCGGTGAGCGATCTGCGTCCGCTGCCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.73
A4	BG_SM_DENOVO_STX_E2E_boltzgen_binder_002	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGAAGTGCGTGTGGGCGATCGTGTGCTGGCGAAAAACAGCGATGGCAACTGGTATGAAGCGGTGATTGAAGAAGTGCTGGATGAAGAAACCGTGAAAGTGCGTTTTCTGGATAACGGCAGCACCAGCGTGGTGAAACTGAAAGATGTGCGTCCGCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.42
A6	BG_SM_DENOVO_STX_E2E_boltzgen_binder_020	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCCTGGTGAAACCGGGCGTGACCCCGGATGGCCGTCGTGGCTGCATTGCGCTGCGTGATATTCCGGGCAGCGGCCCGGGCGGCCGTACCATTAAAAAAGGCGATGTGATTCTGGATGTGCTGGATGATACCACCGTGAAAGTGTATAGCCCGAGCACCAAAAAAGTGATTGAAGCGCGTATTGGCGATCCGGCGGCGATTGCGGAAATTCGTCGTTGCCTGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.35
A2	BG_SM_DENOVO_STX_E2E_boltzgen_binder_011	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGGAATTTAAAGTGGGCGATCGTGTGCTGGCGGAAATTGATGGCAAATGGCATCTGGGCACCATTACCGAAATTCTGGATGATGATACCGCGAAAGTGCTGGTGGATGGCAAAGAATATGAAGTGCGTCGTAGCAACCTGGTGAAAGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.33
A3	BG_SM_DENOVO_STX_E2E_boltzgen_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGGAATTTCGTGTGGGCGATCGTGTGGTGGATCTGAGCCGTGGCGGCGCGGTGGGCGAAGTGGTGGAAGTGCTGGATGATAACACCGTGAAAGTGCAGTATCTGGATGATGGCAGCGAAGCGGTGGTGCCGATTGCGGATCTGGTGCCGGCGAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.30
A5	BG_SM_DENOVO_STX_E2E_boltzgen_binder_003	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGAAAAAGTGAAAGTGGGCGATAAAGTGCTGGCGAAAATTAACGGCAAATGGTATCCGGCGACCGTGGTGGAAGTGCTGGATGATGAAAAAGCGAAAGTGAAATTTGATGAAAACGGCAGCACCGCGGTGGTGCATCTGAAAGATCTGCGTCTGGTGGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.18
A7	BG_SM_DENOVO_STX_E2E_boltzgen_binder_008	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCCGCAGGTGATTGTGCGTGCGCTGCGTGATTATGTGGCGAAAACCGAAAACGAACTGAGCGTGAAAAAAGGCGATCGTCTGCAGATTATTGATCAGCCGAACGAAGATTGGTGGAAAGCGCAGAGCCTGACCACCGGCGAAACCGGCTATATTCCGAAAAGCTATGTGGAAGAAGTGCCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.10
A8	BG_SM_DENOVO_STX_E2E_boltzgen_binder_004	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGAAATATAAAGTGGGCGATGTGGTGCTGGCGCGTGCGAGCAGCGGCGAAGTGTATCGTGCGCGTGTGCTGGAAGTGCTGGATGATGAAACCGTGAAAGTGCAGTTTCTGGAAAACGGCGAAACCGGCGTGCGTCATGTGGCGGATCTGGCGCCGGCGCCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.01
A9	BG_SM_DENOVO_STX_E2E_boltzgen_binder_012	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCCGCGTGTGACCACCCTGGCGGAACTGGCGAAAGCGGGCGCGGGCCCGGGCCTGGTGGTGCGTGTGGTGCGTGTGGCGCCGGGCCTGCCGCTGCGTGAAGGCGATGTGGTGGAAATTGTGGAAGTGCTGGATGAAAACACCGCGGTGGTGCGTAGCCTGGCGACCGGCGAAGAATATCTGGTGAACGCGAAAGATGTGCTGGCGGAACCGCTGACCCCGCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.16
A10	BG_SM_DENOVO_STX_E2E_boltzgen_binder_014	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGTGGTGGTGAAAGATTATACCGCGACCCAGCCGAACGAAATTAGCGTGAAAAAAGGCGATAAAGTGGTGGGCGAAGCGGGCGATGGCTGGGTGCTGGTGCAGGCGGCGGATGGCCGTACCGGCTATGTGCCGGCGAAATATGTGGAACCGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.26
A11	BG_SM_DENOVO_STX_E2E_boltzgen_binder_005	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCCGATTCTGGCGAAAGCGCTGAAAGATTATGAAGGCCCGCGTCCGGGCGAACTGGCGTTTAAAAAAGGCGATATTCTGGAAGTGCTGGAAGTGTATAGCGATGGCAAATATTGGCTGTGCAAAGTGAACGGCAAAACCGGCTGGATTCCGAGCAACTATGTGAAACTGCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.39
A12	BG_SM_DENOVO_STX_E2E_boltzgen_binder_018	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCTGAAAGTGGGCGATAAAGTGGAAGCGACCACCAGCAGCGGCGAAAAACGTATTGGCACCATTACCAGCATTGATGAAGAAAAAAACACCGCGACCATTAAAGTGGATGGCAAACTGGAAACCGTGAGCATTAAAGATCTGAAAAAAGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.41
B2	BG_SM_DENOVO_STX_E2E_boltzgen_binder_009	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCCGCCGATTATTGCGCGTGCGCTGAAAGATTATCTGGCGAAAACCGAAAACGAACTGAGCTTTAAAAAAGGCGATACCCTGGAAATTCTGGAAGTGCCGGATGATGAATGGTGGAAAGCGCGTAGCCTGAACACCGGCGAAACCGGCTATGTGCCGGCGAGCGATGTGGAAGTGGTGGAAGCGGGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.42
B4	BG_SM_DENOVO_STX_E2E_boltzgen_binder_007	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCAAACTGGTGAAAGCGCTGAAAGATTATACCGCGAAAAGCCCGAGCGAACTGAGCTTTAAAAAAGGCGATATTCTGGAAATTCTGGATGATAGCGATCCGACCTGGAGCCTGGCGAAAAGCCTGAACACCGGCGAAGTGGGCTGGTTTCCGACCAGCCTGGTGGAAGAAGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.46
B1	BG_SM_DENOVO_STX_E2E_boltzgen_binder_013	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCAAAAAACTGGTGAAAGCGCTGAAAGATTATACCGCGAAAAGCAGCCATGAACTGAGCTTTAAAAAAGGCGATATTCTGACCCTGCTGGAAGAACTGGATGATAAATGGTGGCTGGCGGAAAAAAACGGCAAAAAAGGCCTGATTCCGAAAAGCAACGTGGAAGAAATTTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.54
B5	BG_SM_DENOVO_STX_E2E_boltzgen_binder_006	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGTGAAAGTGGGCGATATTGTGCTGGCGCGTGCGCCGGATGGCAAAGTGTATAAAGCGGAAGTGCTGGCGGTGGAAGGCGATGAAGCGACCCTGAAATTTCTGGAAAACGGCGAAGTGGAACGTGTGCCGCTGAGCGATATTCTGGCGAAACTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.62
B6	BG_SM_DENOVO_STX_E2E_boltzgen_binder_019	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGCTGGAAGTGGGCGATATTGTGAAAGCGCTGAAAGATTATACCGCGAAAAACAGCAGCGAACTGAGCTTTAAAAAAGGCGATAAACTGCTGGTGCTGGATGTGCCGGATGAAAACTGGGTGAAAGCGCAGAGCCTGGAAACCGGCAAAGTGGGCTGGATTCCGAGCAGCCTGGTGGAAATTGTGGGCCGTGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.65
B3	BG_SM_DENOVO_STX_E2E_boltzgen_binder_017	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGAAACCGACCGAACTGAAAGTGGGCGATATTGTGATTGCGCTGAAAGATTATACCGCGACCAAACCGGAAGAACTGAGCCTGAAAAAAGGCGATAAACTGAAAGTGCTGGATGTGCCGGATGAAAACTGGGTGAAAGCGCAGGTGCTGAGCACCGGCGAAACCGGCTATATTCCGAAAAAATATGTGAAAAAAGCGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.74
B7	BG_SM_DENOVO_STX_E2E_boltzgen_binder_010	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGGAATTTAAAGTGGGCGATATTGTGAAAGCGCTGAAAGATTATATTGGCAAAACCCCGGAAGAACTGAGCTTTAAAAAAGGCGATAAACTGCTGGTGGAAGATGTGCCGGATGATGAATGGATTCTGGCGCGTAGCCTGAGCACCGGCGAAACCGGCTATGTGCCGAAAAAAAACGTGGAAAAAGTGGGCAGCGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.82
B8	BG_SM_DENOVO_STX_E2E_boltzgen_binder_015	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGGAAGGCACCATTGTGAAAGCGCTGAAAGATTATACCGCGAAAACCAGCAGCGAACTGAGCTTTAAAAAAGGCGAACTGCTGGCGGTGCTGGAAGAACTGGATGAAGATTGGTGGAAAGGCCGTAGCCTGGAAACCGGCGAAACCGGCGTGTTTCCGAAAAGCTATGTGGAAGTGGTGAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.88

Yeast Surface DisplayAvailableGenerator filter: passed

Yeast surface display screen — Saxitoxin binder oligo pools

Campaign: saxitoxin--bg-sm-denovo-stx-e2e · Target slug: Saxitoxin

IDT oPools order, tiered by confidence: tier1_50pmol (20 oligos). Gap-repair cloning into pCTCON2.

Binders measured

120

Top Pool score